proB [2017-07-30 17:24:08]

glutamate 5-kinase

Locus: BSU13120

Isoelectric point: 6.01

Molecular weight: 39.30 kDa

Protein length: 365 aa

Gene length: 1095 bp

Function: biosynthesis of proline

Product: glutamate 5-kinase

Essential: no

E.C.: 2.7.2.11

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of proline

Gene

Coordinates: 1,378,496 → 1,379,593

Phenotypes of a mutant

• auxotrophic for proline PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

Protein family

• PUA domain (according to Swiss-Prot)

Paralogous protein(s)

• ProJ

Effectors of protein activity

• the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proB-proA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: anti-termination, in T-box
  • YlxR: regulation, PubMed, in YlxR regulon

  Regulation

  • induced by proline limitation (T-box) PubMed
  • expression requires functional YlxR PubMed

  Additional information

  • ProA is absent from the membrane proteome of a 'BdbC-BdbD' mutant PubMed
  • the T-box RNA is degraded by RNase Y PubMed

References

Reviews

Loading

Original publications

Loading